DNA Services

Please use this form to request strains from the BGSC. Users may enter a purchase order number in the form below, or may call 1-614-292-5550 between 8 am - 5 pm Eastern Time to order with a credit card. Non-profit users will not be turned away due to an inability to pay. BGSC strains, plasmids, and phages are $175 each to for-profit users, $49 each to academic or non-profit users. Prepaid subscriptions are $2650 for up to 50 strains to for-profit users, or $265 for up to 20 strains to academic or non-profit users. Inquire for details. Please note that terms are Net 30. For-profit users are required to supply either a purchase order number (online or via email, fax, or phone) or pay by credit card (phone only) before the order can be shipped.

For international users: some customs departments require a reference number, such as a Tax code, VAT number, or importer code. If this requirement applies to you, please enter the appropriate code above. Strains sent to academic or other non-profit users are normally shipped via USPS first class (airmail if sent outside the US). Strains sent to for-profit users are sent via an express courier. Non-profit users may up-grade to express delivery if they provide us with a FedEx account number to bill.

Do you have any comments or questions? If so please contact us at director@bgsc.org

Genomic DNA Purification

The Bacillus Genetic Stock Center is pleased to offer high molecular weight, highly purified genomic DNA at a very reasonable price. We can supply genomic DNA from any strain in our collection, or from a strain that you provide. We routinely use our genomic DNA for a variety of downstream applications: PCR amplification, direct genomic DNA sequencing, and restriction enzyme mediated cloning. We also offer an inexpensive, partially purified grade of genomic DNA that works quite well as a PCR template in our hands. Note: Due to the cost of refrigerated shipping, we are currently offering this service only to customers within the United States and Canada.

Price Schedule:

Service Quantity price
Genomic DNA, template grade 0.5 ml $165
Genomic DNA, column purified 50-100 µg $189
Shipping per package $45 to USA; $55 to Canada
Download Genomic DNA Order Form

16S rDNA Sequence Determination

The BGSC also has considerable expertise in 16S rDNA Sequence Determination. If you send us a strain (or specify a strain in our collection), we will provide you with the specified 16S rDNA sequence, together with electropherograms and sequence reads for each individual primer used and a taxonomic identification for the strain. Please contact Dan Zeigler at +1-614-292-5550 to discuss your project before submitting any strains for sequence determination.

Price Schedule:

Sequence Returned 1-9 10-19 20-39 >40
First ~500 bp $110 $100 $95 $80
First ~1000 bp $150 $120 $110 $100
Full length $230 $190 $175 $160

Other Requirements

We can provide genomic DNA or 16S rDNA sequence from any strain in our collection. We can also provide these same services for any culture you would send us that meets the following criteria

  • The strain must fall within one of two families, the Bacillaceae or the Paenibacillaceae. These families include dozens different genera and hundreds of species with official standing in the nomenclature. For a listing, please consult: the NCBI taxonomy browser. Any gram-positive endospore-forming isolate that can grow aerobically is assumed to qualify.
  • The strain must be a biosafety level 1 organism. No known pathogen, etiologic agent, or otherwise hazardous organism will be accepted. Bacillus anthracis is absolutely excluded.
  • The strain must be culturable under aerobic conditions using readily available biological growth medium components. You will need to inform us up front about any unusual temperature, pH, osmotic, or nutrient requirements.
Revised 15 July 2010