Genomic DNA Purification
The Bacillus Genetic Stock Center is pleased to offer high molecular weight, highly purified genomic DNA at a very reasonable price. We can supply genomic DNA from any strain in our collection, or from a strain that you provide. We routinely use our genomic DNA for a variety of downstream applications: PCR amplification, direct genomic DNA sequencing, and restriction enzyme mediated cloning. We also offer an inexpensive, partially purified grade of genomic DNA that works quite well as a PCR template in our hands. Note: Due to the cost of refrigerated shipping, we are currently offering this service only to customers within the United States and Canada.
|Genomic DNA, template grade||0.5 ml||$39 non-profit; $139 for-profit|
|Genomic DNA, column purified||50-100 µg||$69 non-profit; $169 for-profit|
|Genomic DNA, column purified||250-500 µg||$79 non-profit; $179 for-profit||Shipping||per package||$45 to USA; $55 to Canada|
16S rDNA Sequence Determination
The BGSC also has considerable expertise in 16S rDNA Sequence Determination. If you send us a strain (or specify a strain in our collection), we will provide you with the specified 16S rDNA sequence, together with electropherograms and sequence reads for each individual primer used and a taxonomic identification for the strain. Please contact Dan Zeigler at +1-614-292-5550 to discuss your project before submitting any strains for sequence determination.
|First ~500 bp||$110||$100||$95||$80|
|First ~1000 bp||$150||$120||$110||$100|
We can provide genomic DNA or 16S rDNA sequence from any strain in our collection. We can also provide these same services for any culture you would send us that meets the following criteria
- The strain must fall within one of two families, the Bacillaceae or the Paenibacillaceae. These families include over twenty different genera and hundreds of species with official standing in the nomenclature. For a listing, please consult: the NCBI taxonomy browser. Any gram-positive endospore-forming isolate that can grow aerobically is assumed to qualify.
- The strain must be a biosafety level 1 organism. No known pathogen, etiologic agent, or otherwise hazardous organism will be accepted. Bacillus anthracis is absolutely excluded.
- The strain must be culturable under aerobic conditions using readily available biological growth medium components. You will need to inform us up front about any unusual temperature, pH, osmotic, or nutrient requirements.