BGSC - Order

Strains

Please use this form to request strains from the BGSC. Strains and plasmids are 179.00 USD each to for-profit users, 55.00 USD each to academic or non-profit users. Prepaid annual subscriptions are 2850.00 USD for up to 50 strains to for-profit users, or 285.00 USD for up to 20 strains to academic or non-profit users. The shipping rate for destinations within the United States is 15.00 USD, while the rate for international destinations is 60.00 USD. For international users paying via wire transfer, an additional $25 wire transfer fee will be added to your invoice. Unpaid balances will result in future orders being held until payment is received. Unpaid invoices will be transferred to the Ohio State University’s collections department after 60 days past the due date.

Users may enter a purchase order number in the form below or may request an online link to complete payment using a credit card. Payment (PO or credit) must be received before the order can be shipped. Also, please remember to enter the strain ID or subscription type in the "Strain Request" field and note that terms are Net 30. Please send inquiries to asc-microbioservice@osu.edu

Strain Request
(Press return, comma, semicolon, tab, or space between strains)

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For international users: Most customs departments require a reference number, such as a Tax code, VAT number, or importer code. If this requirement applies to you, please enter the appropriate code above. If additional customs documents are needed, please email asc-microbioservice@osu.edu with the templates for the appropriate documents.

Shipping and Wire Fees

Shipping Information: Strains are shipped via UPS Express for destinations within the United States and FedEx for destinations outside the United States. Shipping fees for domestic locations is 15.00 USD and 60.00 USD for international locations. For subscription users, the shipping fee is paid once at the start of the subscription.

Wire Payment Information: For international users paying via wire transfer, an additional $25 wire transfer fee will be added to your invoice via wire transfer.

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Do you have any comments or questions? If so please contact us at director@bgsc.org

Genomic DNA Purification

The Bacillus Genetic Stock Center is pleased to offer high molecular weight, highly purified genomic DNA at a very reasonable price. We can supply genomic DNA from any strain in our collection, or from a strain that you provide. We routinely use our genomic DNA for a variety of downstream applications: PCR amplification, direct genomic DNA sequencing, and restriction enzyme mediated cloning. We also offer an inexpensive, partially purified grade of genomic DNA that works quite well as a PCR template in our hands. Note: Due to the cost of refrigerated shipping, we are currently offering this service only to customers within the United States and Canada.

Price Schedule:

Service	Quantity	price
Genomic DNA, template grade	0.5 ml	$165
Genomic DNA, column purified	50-100 µg	$189
Shipping	per package	$45 to USA; $55 to Canada

Download Genomic DNA Order Form

16S rDNA Sequence Determination

The BGSC also has considerable expertise in 16S rDNA Sequence Determination. If you send us a strain (or specify a strain in our collection), we will provide you with the specified 16S rDNA sequence, together with electropherograms and sequence reads for each individual primer used and a taxonomic identification for the strain. Please contact Dan Zeigler at +1-614-292-5550 to discuss your project before submitting any strains for sequence determination.

Price Schedule:

Sequence Returned	1-9	10-19	20-39	>40
First ~500 bp	$110	$100	$95	$80
First ~1000 bp	$150	$120	$110	$100
Full length	$230	$190	$175	$160

Other Requirements

We can provide genomic DNA or 16S rDNA sequence from any strain in our collection. We can also provide these same services for any culture you would send us that meets the following criteria

The strain must fall within one of two families, the Bacillaceae or the Paenibacillaceae. These families include dozens different genera and hundreds of species with official standing in the nomenclature. For a listing, please consult: the NCBI taxonomy browser. Any gram-positive endospore-forming isolate that can grow aerobically is assumed to qualify.
The strain must be a biosafety level 1 organism. No known pathogen, etiologic agent, or otherwise hazardous organism will be accepted. Bacillus anthracis is absolutely excluded.
The strain must be culturable under aerobic conditions using readily available biological growth medium components. You will need to inform us up front about any unusual temperature, pH, osmotic, or nutrient requirements.

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Strains

Genomic DNA Purification

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16S rDNA Sequence Determination

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Other Requirements

Revised 15 July 2010