Welcome


Welcome to the Bacillus Genetic Stock Center (BGSC) web site.

Our primary mission is to maintain genetically characterized strains, cloning vectors, and bacteriophage for the genus Bacillus and related organisms and to distribute these materials without prejudice to qualified scientists and educators throughout the world. Since 1978, the National Science Foundation has funded the activities of the BGSC (Award Number:1756219). The Department of Microbiology in the College of Arts and Sciences at The Ohio State University provides facilities and administrative support. The Director of the BGSC is Dr. Daniel R. Zeigler.


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News

pminiMAD, a tool for markerless allele replacement

What makes Bacillus subtilis 168 such a powerful model system? Many factors: a world-wide community of investigators and a 60-year history of focused enquiry; a carefully annotated genome sequence with associated proteomic and transcriptomic data; a suite of useful curated databases making all of this information discoverable and accessible. But what sets B. subtilis apart from most other microbial model systems is a genetic toolbox of great sophistication and variety. One important set of tools are designed to introduce marker-free mutations to the B. subtilis genome. Several technologies are available, including Cre-lox marker loop-out and CRISPR methodologies. But an older

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