BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
---|---|---|---|
BEC11340 | Bacillus subtilis subsp. subtilis | Cm Em trpC2 lacA::Pxyl-dcas9 amyE::Pveg-sgRNA(fabF) |
BEC11340 CAG74640 |
Shipping Conditions: Nutrient agar #2 (Oxoid) with chloramphenicol (5 µg/ml) and erythromycin (1 µg/ml) | |||
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium containing chloramphenicol (5 µg/ml) and erythromycin (1 µg/ml) | |||
Temperature: 37 | |||
References: Stork DA, Squyres GR, Kuru E, Gromek KA, Rittichier J, Jog A, Burton BM, Church GM, Garner EC, Kunjapur AM. Designing efficient genetic code expansion in Bacillus subtilis to gain biological insights. bioRxiv 2021.02.19.432053; doi: https://doi.org/10.1101/2021.02.19.432053 |