New Bacillus subtilis strain containing SPB phage

The BGSC is pleased to announce that the phage containing strain CMJ114 is now available in our collection. CMJ114 is a B. subtilis strain that contains the temperature-sensitive SPBeta lysogen with a wild type yonE allele.
This strain can be found under the BSGC accession number 1L57.

Thank you to Janet Smith from the Grossman lab at MIT for generously donating this strain!

ICE Mobile-CRISPRi transformation strains

The BSGC is pleased to provide two strains from a suite of CRISPRi systems that combines modularity, stable genomic integration and ease of transfer to diverse bacteria by conjugation. These strains are CAG80612 (BGSC ID 1A1640) and CAG81072 (BGSC ID 1A1641) and were generously donated by Dr. Jason Peters from the University of Wisconsin-Madison.

New Bacillus SANDBOX Plasmids

The BGSC is pleased to announce the availability of five new integration vectors, pBSAND1, pBSAND2, pBSANDdel, pBSANDlux, and pBSGGlux. These plasmids are available under the accession numbers ECE801-ECE805.

The plasmid designated pBSAND1 (ECE801) can be linearized using ScaI to allow for integration in B. subtilis at thrC. The plasmid designated pBSAND2 (ECE802) can be linearized using NgoMIV to allow for integration in B. subtilis at lacA. The plasmid pBSANDdel (ECE803) cuts upstream of the gene rsoI and allows for removal of the entire sigO-rsoA operon in B. subtilis W168. When utilised with plasmids pBSAND1-PliaI and pBSAND2-PxylA, pBSANDlux (ECE804) allows for bacitracin and xylose inducible luciferase output. The plasmid pBSGGlux (ECE805) can be linearized using ScaI for integration in B. subtilis at the sacA locus.

Thank you to Susanne Gebhard at University of Bath for generously donating these plasmids to our collection.

New Markerless Transposons for Fluorescent Fusion

The BGSC is pleased to announce the availability of the new TnFLX transposon system in Bacillus subtilis. The plasmids, which are available in the catalog as ECE797-ECE800, can be used to generate internal markerless fusions to fluorescent protein reporters. These plasmids can be used to produce protein fusions in Bacillus subtilis that introduce fluorescence and preserve functionality. This is a useful tool for investigating protein localization in vivo.

Thank you very much to the Daniel Kearns lab at Indiana University for donating these materials to our collection.