News

From the laboratory of Wolfgang Schumann at the University of Bayreuth come two new expression vectors capable of integrating into the Bacillus subtilis chromosome at the lacA locus. Each allows for regulated expression of cloned inserts.

Bacillus subtilis Integration Vectors with Inducible Expression of Cloned Inserts

pMUTIN4 should allow the researcher to produce knockout or conditional expression mutations in any unknown coding sequence.

A Vector Useful for Gram-Positive Genomics

From the laboratory of Chet Price at UC Davis comes an important set of mutants altered with the regulation of late stationary phase events. PrpC is a member of the PPM family of serine/threonine protein phosphatases; it removes phosphates from PrkC, a serine/threonine kinase. The pair is believed to regulate the activity of Elongation factor G (EF-G) during stationary phase. In prpC knockouts, such as PB702 (=BGSC 1A961), stationary phase cultures grow to a much greater density in rich, non-sporulation media. In contrast, stationary phase cultures of prkC knockouts, such as PB705 (=BGSC 1A962), grow to a significantly lower density. In double mutants, such as PB722 (=BGSC 1A964), prkC is epistatic to prpC (1). We thank Tatiana Gaidenko and Chet Price for donating this set of strains to the BGSC.

Neil Welker has donated plasmid pNW33N, a fifth generation vector that stably replicates in Bacillus subtilis, Geobacillus stearothermophilus and Escherichia coli

Cloning Vector for Thermophilic Bacillus Strains