Welcome to the Bacillus Genetic Stock Center (BGSC) web site.

Our primary mission is to maintain genetically characterized strains and cloning vectors for the genus Bacillus and related organisms and to distribute these materials without prejudice to qualified scientists and educators throughout the world. The National Science Foundation funded the activities of the BGSC (Award Number:1756219) starting in 1978 and BGSC is no longer funded by NFS. The Infectious Disease Institute at The Ohio State University provides facilities and administrative support.

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New Markerless Transposons for Fluorescent Fusion

The BGSC is pleased to announce the availability of the new TnFLX transposon system in Bacillus subtilis. The plasmids, which are available in the catalog as ECE797-ECE800, can be used to generate internal markerless fusions to fluorescent protein reporters. These plasmids can be used to produce protein fusions in Bacillus subtilis that introduce fluorescence and preserve functionality. This is a useful tool for investigating protein localization in vivo.

Thank you very much to the Daniel Kearns lab at Indiana University for donating these materials to our collection.

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The Bacillus Genetic Stock Center

Biological Sciences 556

484 W. 12th Ave

Columbus, OH 43210-1214

Email: ASC-microbioservice@osu.edu

Accessibility Information 

This material is based upon work supported by the National Science Foundation under Award No. 0742066.

Opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the NSF.

Even the author isn’t all that impressed with his opinions, for that matter!