Welcome to the Bacillus Genetic Stock Center (BGSC) web site.

Our primary mission is to maintain genetically characterized strains and cloning vectors for the genus Bacillus and related organisms and to distribute these materials without prejudice to qualified scientists and educators throughout the world. The National Science Foundation funded the activities of the BGSC (Award Number:1756219) starting in 1978 and BGSC is no longer funded by NFS. The Infectious Disease Institute at The Ohio State University provides facilities and administrative support.

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New Bacillus SANDBOX Plasmids

The BGSC is pleased to announce the availability of five new integration vectors, pBSAND1, pBSAND2, pBSANDdel, pBSANDlux, and pBSGGlux. These plasmids are available under the accession numbers ECE801-ECE805.

The plasmid designated pBSAND1 (ECE801) can be linearized using ScaI to allow for integration in B. subtilis at thrC. The plasmid designated pBSAND2 (ECE802) can be linearized using NgoMIV to allow for integration in B. subtilis at lacA. The plasmid pBSANDdel (ECE803) cuts upstream of the gene rsoI and allows for removal of the entire sigO-rsoA operon in B. subtilis W168. When utilised with plasmids pBSAND1-PliaI and pBSAND2-PxylA, pBSANDlux (ECE804) allows for bacitracin and

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The Bacillus Genetic Stock Center

Biological Sciences 556

484 W. 12th Ave

Columbus, OH 43210-1214

Email: ASC-microbioservice@osu.edu

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This material is based upon work supported by the National Science Foundation under Award No. 0742066.

Opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the NSF.

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